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Phytochemical estimation, antioxidant and antimicrobial properties of Pleocaulus sessilis: an endemic plant from Western Ghats of India Scientia Research LibraryJournal of Applied Chemistry2348 - 0408201535 Sujata V. Patil1, Rahul P. Mane1, P. V. Anbhule1,2 and V. B. Shimpale1,3* M. B. Deshmukh1,2 PageNo. 1-10 1 The phytochemical constituents antioxidant and antimicrobial activities of extracts of Pleocaulus sessilis using different solvents were evaluated. The dried powdered leaves of Pleocaulus sessilis were extracted with acetone, ethanol and methanol. All extracts were analyzed by high performance liquid chromatography (HPLC) to estimate the compound content. The phytochemicals were estimated according to the standard methods.1,1-diphenyl-2-picrylhydrazyl,Hydrogen peroxide and reducing power assay were used to determine in vitro antioxidant and antimicrobial activities and were performed using standard protocol. Phytochemical analysis of extracts showed presence of main group phytochemicals such as phenols, flavonoids, saponins and tannins. Methanolic extract was found to contain the highest phenolic and flavonoid content. In vitro antioxidant activities and antimicrobial activities of all extracts were found to be good. On the basis of the results obtained, all the varied extracts of Pleocaulus sessilis seems to be the most capable species for further exploration in order to identify the chemical ingredients responsible for their activity. Keywords: flavonoid content, phenolic content, RP- HPLC, Antioxidant activity, Pleocaulus sessilis. http://www.scientiaresearchlibrary.com/archive-abs.php?arc=378http://www.scientiaresearchlibrary.com/archive/JAC-2015-3-5-063-1-10.pdf